On these pages you will find useful information to help you use the microscopes at the NIC@King's and process the resulting data. We have included a set of "user guides" for the microscopes at the centre as well as some official Nikon technical notes about the use of NIS-Elements for processing microscopy images. We're also fans of open source software and use ImageJ, CellProfiler and Icy to complement NIS-Elements. We hope to build up a repository of information which we hope will be useful in helping in all aspects of the light microscopy pipeline - all the way from image acquisition to publication.