Professor Maddy Parsons - NIC Director

Dr Daniel Matthews - NIC Manager

Miss Isma Ali - NIC Deputy Manager

Dr Ben Robinson - NIC Deputy Manager

Nikon and King's

The NIC@King's is a partnership with Nikon and is one of nine centres globally

3rd Floor Hodgkin Building - Guy's Campus

NIC@King's

  • Opened April 2012, over 200 trained users
  • Overseen by steering committee made up of academic members of staff
  • Users charged for all systems - cost determined by system maintenance contract (see http://nic.kcl.ac.uk/access/charges/)

Biomedical research at King's

  • Cancer - cell biology (cytoskeleton, adhesion, migration, invasion), stem cell biophysics, in vivo models
  • Neurobiology - in vivo developmental models, intracellular traffic, synapse assembly
  • Cardiovascular – regulation of cardiac myocytes, heart development in vivo, drug delivery
  • Asthma and allergy – inflammatory cell migration, apoptosis,drug screens
  • Immunology – immune cell traffic (in-vitro, in-vivo), virus assembly and infection

Training/Demos/Workshops

  • We provide one-to-one training for new users on each required instrument and on-going day-to-day support
  • Masters and PhD workshops: combined lectures and hands-on practical training
  • External (non-KCL) collaborators and users (industry and academia)
  • Showcase site for Nikon for potential customers
  • Training site for Nikon staff
  • Beta-testing for new Nikon products

Instruments

Currently 10 systems

  • 2x inverted point scanning confocals
  • 1x upright point scanning confocal
  • 1x upright point scanning multiphoton
  • 2x widefield fluorescence, live cell systems
  • 1x N-STORM
  • 1x N-SIM
  • 1x inverted spinning disk confocal
  • 1x Biostation IMQ

A1R Confocal

  • Configured for speed and sensitivity
  • Resonant scanner and piezo z-drive
  • GaAsP high sensitivity detectors
  • Can be used for live or fixed samples

A1R Confocal

Upright Confocal

  • Owned by Prof Fiona Watt
  • Imaging of stem cells and mouse skin/hair follicles
  • Shared usage with other centre users (~70/30 split)

Image courtesy of Kif Liathakula - hair folicles in mouse epidermis

A1R Upright Multi-photon

  • Good for deep tissue imaging ( including in-vivo)
  • Resonant scanner and piezo z-drive for high-speed imaging
  • Can also be used as a standard upright confocal

Spinning disk confocal

  • Variety of projects – lots of live cell imaging
  • Inverted SDC has NEO sCMOS

Biostation

  • Microscope in-a-box for very long term imaging
  • Variety of projects: T-cell interactions/wound healing
  • Popular because of low maintenance and ease of use

Live cell imaging

  • 2x live cell widefield systems
  • Both systems have an sCMOS monochrome camera, system 2 also has an RGB camera for H&E samples

Structured Illumination - NSIM

  • 2-fold improvement in spatial resolutuon compared with confocal
  • Also configured for TIRF-SIM
  • Stage-top incubator for live cell imaging

NSTORM

  • 20-40nm resolution in XY
  • Capable of nSTORM, dSTORM and PALM

dSTORM (Alexa647) tubulin

Two colour nSTORM

3D nSTORM

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