N-SIM super resolution

nsim scopeSuper resolution - Imaging at double the resolution of a widefield microscope ~120nm or ~85nm in TIRF-SIM mode.

Live cell imaging - super resolution imaging at ~ 1fps or ~0.6fps in TIRF-SIM and 2D-SIM mode

Microscope Eclipse Ti-E Inverted
Imaging System Andor iXon EMCCD
Objectives 10x, 60x water and 100x oil.
Lasers 405nm, 488nm, 561nm, 640nm
Fluorescence Observation DAPI, FITC, TRITC
Environment Tokai-Hit stage-top incubator